Abstract Roadkill is one of the most direct impacts on fauna, leading to numerous direct and indirect impacts. Road-killed animals could serve as a resource for research and teaching purposes, but first, they need to have their tissues preserved by some technique. Plastination is one of the most modern techniques for the conservation of biological tissues, its principle is the replacement of body fluids by a curable polymer, resulting in a non-toxic, odorless, and manipulable material. So the main aim of this work was to compose a collection of plastinated roadkill victims from the Biological Reserve Sooretama region, adapting the standard protocol to the different taxa worked here (Avialae, Reptilia, Amphibia, and Mammalia). For this, 42 animals were used, previously frozen and stored in the Sooretama Biological Reserve and Vale Natural Reserve until they were sent for processing. Upon arriving at the Plastination laboratory at the Federal University of Espírito Santo, the animals underwent the 4 stages of plastination: fixation, dehydration, forced impregnation, and chemical cure; some adaptations were made to improve the final specimens. The results proved that the plastination of these animals is extremely viable in generating satisfactory results. Given the results obtained, it becomes clear how plastination can become an alternative to implement in the composition of biological collections using roadkill animals. The animals prepared in this work comprise an unprecedented collection available for different applications, such as exhibitions, practical classes, and research.

Resumo Estruturas de concreto armado devem ser projetadas de maneira a garantir um nível aceitável de segurança e desempenho. Porém, acidentes ainda são observados atualmente causando interdições, perdas financeiras, e até mesmo humanas. Portanto, estudos aprofundados são necessários para responder a essas situações indesejáveis, prevenindo sua repetição e desenvolvendo novas formas de dimensionamento e construção. O presente trabalho analisa o mecanismo de falha observado em um extenso painel de concreto armado composto por uma parede em balanço apoiada em um pilar parede, localizados na cidade de Campinas. As investigações foram realizadas in loco utilizando testes destrutíveis e não destrutíveis e também via simulações numéricas. Os testes in loco revelaram que a região onde ocorreu a ruptura localizada não possuía quantidade suficiente de armadura metálica. A linha de fratura se desenvolveu no término das armaduras horizontais da parede. Concluiu-se que a falha do painel ocorreu devido a erros de concepção e dimensionamento, dado que os estribos do pilar-parede foram responsáveis pelo equilíbrio da parte em balanço da edificação, como ficou demonstrado pelo estudo utilizando análise não linear via Elementos Finitos e pelas investigações in-loco. desempenho Porém interdições financeiras humanas Portanto indesejáveis construção Campinas numéricas metálica Concluiuse Concluiu pilarparede edificação inloco. inloco loco. in-loco

Abstract Reinforced concrete structures must be designed in a manner that assures an acceptable level of safety and performance. However, accidents still occur nowadays causing interdiction, financial losses, or even human casualties. Therefore, succinct studies are required to give answers to such an undesirable situation, helping to prevent repetition and enlightening new design and construction methods. This paper analyzes the failure mechanism in a large reinforced concrete panel composed of one L-shaped cantilevered wall and structural walls in Campinas. The investigation was carried out using in-situ destructive and non-destructive tests and also through numerical simulation. In-situ tests revealed that the region where the fracture happened had no sufficient resisting steel area. The fracture line developed through the end of the wall’s horizontal rebars anchored within the column. It was concluded that the reinforced concrete panel failure happened due to structure misconception and incorrect design, once columns stirrups were responsible for the equilibrium of the cantilevered part of the building, as demonstrated by the nonlinear finite element study and in-situ investigations. performance However interdiction losses casualties Therefore situation methods Lshaped L shaped Campinas insitu situ nondestructive non simulation Insitu In area s column building investigations

Abstract Objective To assess the economic and professional impact (including teaching and training) during the covid-19 pandemic, as well as the prevalence of major depressive episode and generalized anxiety disorder, on shoulder and elbow surgeons in Brazil. Methods Cross-sectional study carried out with surgeons who are members of the Brazilian Society of Shoulder and Elbow Surgery (SBCOC). Sampling was non-probabilistic for convenience. The information was obtained through a self-administered online questionnaire, through the SBCOC members' registered e-mails, using structured questions and the Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder-7 (GAD-7) instruments to screening for major depressive episode and generalized anxiety disorder, respectively. Results 119 participants were evaluated. The commitment in teaching was affirmed by 87.7% of the surgeons, there was a reduction above 50% of the surgical volume for about 4 out of 10 surgeons and reduction of the income above 50% for about 17.3%, due to the fact that 55.5% of professionals did not have professional stability. The major depressive episode had a prevalence of 26.7% in the sample, and generalized anxiety disorder was presented by 20% of the surgeons. Conclusion The combination of the negative impact on professional and financial factorsmay have affected shoulder and elbow surgeons in Brazil, generating high rates of anxiety and depression.

Resumo Objetivo Avaliar o impacto econômico e profissional (incluindo ensino e treinamento) durante a pandemia de covid-19, bem como a prevalência de episódio depressivo maior e de transtorno de ansiedade generalizada, nos cirurgiões de ombro e cotovelo do Brasil. Métodos Estudo transversal realizado com os cirurgiões membros titulares da Sociedade Brasileira de Cirurgia de Ombro e Cotovelo (SBCOC). A amostragem foi não probabilística por conveniência. As informações foram obtidas por meio de questionário online autoaplicado, através de divulgação pelo e-mail cadastrado dos membros da SBCOC, utilizando questões estruturadas e os instrumentos Patient Health Questionnaire (PHQ-9) e Generalized Anxiety Disorder-7 (GAD-7) para rastreio de episódio depressivo maior e transtorno de ansiedade generalizada, respectivamente. Resultados Foram avaliados 119 participantes. O comprometimento no ensino foi afirmado por 87,7% dos cirurgiões, houve redução acima de 50% do volume cirúrgico para cerca de 4 em cada 10 cirurgiões e redução da renda acima de 50% para cerca de 17,3%, sendo que 55,5% dos profissionais não apresentavam estabilidade profissional. O episódio depressivo maior apresentou uma prevalência de 26,7% na amostra, e o transtorno de ansiedade generalizada foi apresentado por 20% dos cirurgiões. Conclusão A combinação do impacto negativo sobre fatores profissionais e financeiros pode ter afetado os cirurgiões de ombro e cotovelo do Brasil, gerando elevados índices de ansiedade e depressão.

Resumo: A biodiversidade alimentar é caracterizada pela diversidade alimentar que compõe um ecossistema local, regional ou nacional. O Brasil tem 20% de toda a biodiversidade do planeta e os biomas mais ricos do mundo. No entanto, é relevante descrever a participação desses alimentos na dieta dos brasileiros. Utilizando uma amostra complexa com dados de 57.920 domicílios, coletados pelo Instituto Brasileiro de Geografia e Estatística de 2017 a 2018, observou-se que, com exceção da erva-mate, a disponibilidade de alimentos provenientes da biodiversidade é baixa, representando uma média de 7,09g/per capita/dia. Por biomas, a Caatinga apresentou a maior disponibilidade de frutos (4,20g/per capita/dia), enquanto para legumes, a Amazônia se destacou (1,52g/per capita/dia). Os resultados são insatisfatórios e abaixo do que se espera de um território biodiverso e de um sistema alimentar que é destaque mundial. É necessário um maior comprometimento para a promoção de ações que fortaleçam o consumo desses alimentos entre brasileiros.

Resumen: La biodiversidad alimentaria se caracteriza por la diversidad alimentaria que conforma un ecosistema local, regional o nacional. Brasil tiene el 20% de toda la biodiversidad del planeta y los biomas más ricos del mundo. Sin embargo, es relevante describir la participación de estos alimentos en la dieta de los brasileños. Utilizando una muestra compleja con datos de 57.920 domicilios, recopilados por el Instituto Brasileño de Geografía y Estadística de 2017 a 2018, se observó que, con excepción de la yerba mate, la disponibilidad de alimentos de la biodiversidad es baja, representando un promedio de 7,09g/per cápita/día. Por biomas, la Caatinga presentó la mayor disponibilidad de frutos (4,20g/per cápita/día), mientras que en relación a las legumbres, la Amazonía se destacó (1,52g/per cápita/día). Los resultados son insatisfactorios y por debajo de lo que se espera de un territorio rico en biodiversidad y un sistema alimentario mundialmente reconocido. Es necesario un mayor compromiso para la promoción de acciones que fortalezcan el consumo de estos alimentos entre los brasileños.

Abstract: Food biodiversity is characterized by the diversity of foods that compose a local, regional, or national ecosystem. Brazil has 20% of all the planet’s biodiversity and the richest biomes in the world. Therefore, describing the participation of these foods in the Brazilian diet is relevant. Using a complex sample with data from 57,920 households collected by the Brazilian Institute of Geography and Statistics from 2017 to 2018, this study showed that, except for yerba mate, the availability of foods from Brazilian biodiversity is low, representing an average of 7.09g/per capita/day. Regarding biomes, the Caatinga had the highest availability of fruits (4.20g/per capita/day) while the Amazon had the highest availability of vegetables (1.52g/per capita/day). The results are unsatisfactory and lower than what is expected from a territory rich in biodiversity and a world-leading food system. A greater commitment is essential to promote actions that strengthen the consumption of these foods among Brazilians.

Abstract Acinetobacter spp. are one of the main pathogens responsible for healthcare-associated infections and are associated with high rates of morbidity and mortality globally, mainly because of their high capacity to present and develop resistance to antimicrobials. To identify species of the Acinetobacter and their resistance profiles from samples collected from hospitalized patients, health professionals and hospital environmental sources in the intensive care units of different public reference hospitals in Porto Velho City, Rondônia, Western Brazilian Amazon. Isolates were identified using microbiological and molecular techniques. The antimicrobial susceptibility profile was determined by disk diffusion. A total of 201 Acinetobacter spp. isolates were identified, of which 47.3% originated from hospital structures, 46.8% from patients and 6% from healthcare professionals. A. baumannii and A. nosocomialis were the most prevalent, with frequency of 58.7% and 31.8%, respectively. Regarding the susceptibility profile, it was observed that 56.3% were classified as multidrug-resistant and 76.2% of the samples belonging to A. baumannii were resistant to carbapenems. In contrast, 96.9% were susceptible to polymyxin B and 91.3% to doxycycline. The data presented here can be used to guide and strengthen the control of multidrug-resistant infections caused by Acinetobacter spp., in addition to improving providing information from a traditionally unassisted region of Brazil.

ABSTRACT Insects are fundamental to biodiversity conservation in almost all ecosystems, and their population decline, and extinction directly result from environmental impacts. These facts are aggravated by the lack of knowledge of insect biodiversity, the so-called biodiversity deficits, especially the Linnean and Wallacean shortfalls. In freshwater ecosystems, biodiversity loss is higher among aquatic insects, and caddisflies comprise one of the most vulnerable orders. In this way, research focusing on describing new caddisfly species and understanding their distribution ranges will increase knowledge of caddisfly biodiversity. In the past 14 years, the team from the Laboratório de Entomologia Aquática “Prof. Dr. Claudio Gilberto Froehlich” (LEAq, Universidade Federal da Bahia, Brazil) and collaborators have been addressing these issues, resulting in 55 described species of caddisflies. Taxa in other insect orders have also received attention and an additional 16 species have been described (eight mayflies, four stoneflies, four neuropteran spongillaflies, and antlions). Here, eight caddisfly species are described and illustrated (Atopsyche froehlichi sp. nov., Austrotinodes zeferina sp. nov., Cernotina kariri sp. nov., Neoathripsodes froehlichi sp. nov., Notalina claudiofroehlichi sp. nov., Oecetis marcus sp. nov., Phylloicus froehlichi sp. nov., and Polycentropus claudioi sp. nov.), five of them in honor of LEAq’s patron. In addition, new distributional data are presented for 10 known species. A checklist of the caddisfly fauna of Bahia state is also presented, with 138 species, around 75% and 30% of them recorded and described by the LEAq team, respectively. Currently, for caddisflies, Bahia is the fifth most species-rich state in Brazil, and the first in the Brazilian Northeast region.

Abstract Background Human Papillomavirus (HPV) is the main etiological factor for the development of cervical cancer. HPV 18 is the second most frequent type, accounting for up to 65% of all cases. HPV intratypic variation may influence the potential for progression to invasive cancer. The aim of this study was to evaluate the prevalence of human papillomavirus 18 intratypic variants in cervical cancer samples from women in the state of Maranhão, Brazil. Methods The study included 118 women over 18 years of age with a diagnosis of cervical cancer. Tumor fragments were collected and subjected to DNA extraction and Polymerase Chain Reaction (PCR) for HPV detection using the PGMY09/11 and GP+5/6 primers. Positive samples were submitted to automated sequencing for viral genotyping. To determine the HPV 18 lineages, positive samples were submitted to PCR, using specific primers to amplify the LCR and E6 regions of HPV 18 virus. Results HPV was present in 88 women (73.3%). Of those, 48 (54%) were HPV 16, the most prevalent, followed by 12 (13.6%) HPV 18. Histologically, squamous cell carcinoma was predominant (79.1%). Among the HPV 18 variants identified, 10 (80%) belonged to lineage A, and sublineages A1, A2, A3, and A4. Two (29%) HPV 18 B variant was also detected, with the sublineages B1 and B2. In this study, the C variant was not found. There was no statistically significant association between the HPV 18 lineages found and sociodemographic and lifestyle variables (p > 0.05). Conclusions A higher frequency of HPV 16 and 18 were found in women with cervical cancer in the state of Maranhão, Brazil, with a high prevalence of the lineage A among women with HPV 18.

Abstract Objective: To describe the development of a website and the creation of a social network account about pediatric allergy/immunology with reliable information, to promote education and have a channel for patient-doctor contact. Methods: This is a descriptive study. A survey was conducted with 93 patients (12 years and older) and caregivers of a Pediatric Allergy/Immunology outpatient clinic, to assess internet usage patterns of potential users. A webpage in Portuguese and an Instagram® account were launched in which it was created an area for patient-doctor communication in the pandemic context. Results: Among 93 participants, 77% were female, 82% caregivers. Median age was 33.2 years, family income 403 dollars/month. The internet was accessed via smartphone by 81,7% of the participants; 76% reported using internet to access health information but 72% did not trust on the information from the internet, and 96% believed that an institutional site could provide meaningful information. From the website release in November 6, 2018 to January 20, 2022, it was counted 10,062 page views by 4,896 users; 55% were 18–34 years old, 70.2% female. Instagram® account gathered 882 followers. Website went through a period of instability during which access were not counted. Due to social isolation during COVID-19 pandemic, the website served as a tool for first response to help patients and doctors. Conclusions: Patients and caregivers of the Pediatric Allergy/Immunology service, consulted about digital tools, considered the information supported by a teaching/research institution timely and relevant. The website and Instagram® account have both performed well and shown good return in relation to hits, and results are continuously being evaluated. During COVID-19 pandemic, the website has been connecting patients/families and doctors.

RESUMO Objetivo: Descrever o desenvolvimento de um website e a criação de uma conta em rede social sobre alergologia/imunologia pediátrica com informações confiáveis, para promover educação e ter um canal de contato paciente-médico. Métodos: Trata-se de um estudo descritivo. Um questionário foi aplicado a 93 pacientes (12 anos de idade ou mais) e cuidadores frequentadores de um ambulatório de alergologia/imunologia pediátrica, para abordar o padrão de uso de internet de potenciais usuários. Lançou-se um website em português e uma página no Instagram®, nos quais foi criada, no contexto da pandemia, uma área para comunicação paciente-médico. Resultados: Dos 93 participantes, 77% eram mulheres, 82% cuidadores, com idade mediana de 33,2 anos e renda mensal de 403 dólares. A internet foi acessada via smartphone por 81,7% dos participantes; 76% relataram usar a internet para informações em saúde, mas 72% não confiavam nessa informação, e 96% acreditavam que um site institucional poderia prover informações seguras. Do lançamento do website em 6 de novembro de 2018 até 20 de janeiro de 2022, contabilizaram-se 10.062 visualizações de páginas por 4.896 usuários; 55% tinham idade entre 18 e 34 anos, 70,2% eram mulheres. A página no Instagram® reuniu 882 seguidores. O website apresentou período de instabilidade, sem registrar acessos. Em razão do isolamento social durante a pandemia de COVID-19, o website apresentou-se como ferramenta de primeira linha para auxiliar pacientes e médicos. Conclusões: Pacientes e cuidadores do ambulatório de Alergologia/Imunologia Pediátrica, consultados sobre ferramentas digitais, consideraram as informações fornecidas por uma instituição de ensino/pesquisa como oportunas e relevantes. O website e a página do Instagram® têm apresentado bom desempenho e visualização, e os resultados estão sendo continuamente avaliados. Durante a pandemia de COVID-19, o website tem conectado pacientes/familiares e médicos.

Abstract The pathogenesis of systemic lupus erythematosus (SLE) is complex. Few studies in Brazilian population have addressed cell phenotypes associated with immunological responses and their associations with SLE activity. The aim of this study is to investigate cell phenotypes associated to SLE diagnosis, treatment and activity. Twenty-eight SLE female patients (17 inactive, 11 active) and 10 healthy women were included in this study. Markers of natural killer (Nk), T and B cells in peripheral blood were evaluated by flow cytometry. Nkt cells were decreased only in SLE active patients. Activated CD4+, regulatory T FoxP3+ and B cells were decreased in both active and inactive SLE patients, compared to control group. The data corroborate the disruption of immune regulatory response in SLE patients and suggest phenotipic changes as possible biomarkers of SLE activity.

ABSTRACT Children have an increased likelihood of becoming carriers of the chronic hepatitis B virus. A total of 1,381 children and adolescents were assessed in five municipalities of Maranhao State, Brazil, for detection of anti-HBc, HBsAg and anti-HBs serologic markers and sociodemographic and behavioral features. Among those who were HBsAg negative and anti-HBc negative, the proportion of anti-HBs positives was calculated after the individuals had completed the vaccination schedule. The robust variance of the Poisson’s regression model was used in order to have adjusted tables and calculate the prevalence ratio. Multivariate analysis was performed to identify the factors associated with the prevalence of anti-HBc with or without HBsAg and the vaccine response. It was observed that 163 children were anti-HBc positive and nine individuals were HBsAg positive. The factors associated with the infection were: municipality of residence (residing in Morros municipality or Humberto de Campos municipality), residence in a rural area, aged between 13 and 15 years old, and illicit drug use. The percentage of individuals who were anti-HBc negative and received all three doses of the vaccine was 48.5%. Among these, only 276 (38.9%) had antibodies at protective concentrations. In an adjusted analysis, Morros municipality presented an increased positivity of vaccine response (p < 0.001), and the age ranging between 6 and 10 years old presented a reduced frequency of response. This study reveals a high prevalence of current and past HBV infection within the targeted age group which, in addition to the low vaccination coverage and serological responses, raises concerns about the management of prevention measures, especially the quality of vaccination in these locations.

Abstract: The article seeks to understand the conjuncture in which we found ourselves when the pandemic hits Brazilian territory, seeking to analyze it’s consequences for social reproduction, both for capital and for workers. The research that resulted in the article is based on bibliographic and documentary studies, guided by the steps of historical-dialectical materialism. It seeks to initially highlight the changes in the world of work that have been implemented since the country's redemocratization process. Next, it seeks to expose elements that pointed to the increase in inequalities and the worsening of the living conditions of workers, in addition to highlighting some of the fractions of the working class especially affected. The structuring elements that impact on social reproduction result from the implemented counter-reforms, and not from the impacts of the pandemic itself; the actions taken to face the crisis aimed at maintaining capital, to the detriment of work.

Resumo: Este artigo busca compreender a conjuntura na qual nos encontrávamos quando a pandemia atinge o território brasileiro, procurando analisar suas consequências para a reprodução social tanto do capital, quanto dos trabalhadores. A pesquisa se pauta em estudos bibliográficos e documental, guiando-se nos passos do materialismo histórico-dialético. Busca evidenciar, inicialmente, as transformações no mundo do trabalho que vêm sendo implementadas desde o processo de redemocratização do país. A seguir, procura expor elementos que apontavam para o aumento das desigualdades e a piora das condições de vida dos trabalhadores, além de evidenciar algumas das frações da classe trabalhadora especialmente afetada. Os elementos estruturantes que impactam sobre a reprodução social resultam das contrarreformas implantadas, e não dos impactos da pandemia em si; as ações tomadas para o enfrentamento da crise objetivavam a manutenção do capital, em detrimento do trabalho.

This paper aims to explore the production of porous Nb2O5 pellets by pressing and adding anaerobic biomass (sludge) in order to evaluate the microstructure porosity. The thermal treatment temperature of the pellets varied between 300 ºC and 900 ºC. Their structural, morphological, and physical properties were measured, and the adsorption potential of Mn2+ and their reuse were evaluated. All samples had a monoclinic phase. Density average values of samples pressed using sludge ranged between 2.45 and 2.52 g.cm-3, while the one obtained without sludge pressing achieved higher values of around 2.72 g.cm-3. All samples presented high porosity values after the heat treatment (above 40%), which confirms the efficiency of sludge as a means to enhance porosity. These pellets were used for Mn2+ ions adsorption in aqueous medium. The Langmuir isotherm model was the one that achieved the best goodness-of-fit. In kinetic studies, the adsorption reaction was in accordance with second-order kinetics. Adsorbents in the form of pellets are environmentally friendly on account of their significant reuse and recycling potential. However, the particulate matter might become an environmental liability and incur high treatment costs. The samples proved to be reusable, promoting the adsorption of 67% of the Mn2+ after the fifth reuse cycle.